Pure Production and Stable Isotopic Labeling of Desired Proteins with the SPP System Elucidation of protein structure and function maintains an important role in post-genomic sequencing and analysis ...

Figure 1: Structures of pCold vectors I, II, III and IV. The present pCold system can also be used for high-level protein production for other biochemical and biophysical studies. For example, the E.

Here, we provide a detailed protocol for the single protein production (SPP) system, which is designed to produce only a single protein of interest in living Escherichia coli cells. Induction of MazF, ...